I am using 10% DMSO to dissolve small componds, however in that higher concentration also they are precipitating. we cannot use more than 5%DNSO in the assay. Please help in dissolving the same.
Please help
You can try dissolving the compound first in 100% DMSO and then do serial dilutions into diH2O. If that doesn't work, try dimethylformamide (DMF)
I could dissolve in 100% but when I did serial dilution with the buffer that i used for the assay, it is getting precipitated. and I tried increasing final concentration to 5%, however no change I could find.
Don't do the serial dilution in buffer, use diH2O. Salts in the buffer decrease solubility. Do the last serial dilution into the buffer. Hopefully by then the compound will be too dilute to precipitate immediately.
Dear Stefansson,
I did the same, I did final dilution with buffer then also compound is not dissolving.
One more question I want to ask here, to check the inhibitor initially what is the maximum Molarity that we have to use.
Bummer.
Check out other solvents such as isoprop., DMF.
What type of assay are you doing? If you are doing cell assays, you could try starting at nM conc. and work your way up. Also for cell assays, you can try diluting the drug using serum or load the drug onto serum albumin.
I am doing in-vitro compitative binding assay with the protein. I am starting with 250uM of compound for the assay and stock is 25mM(dissolved in DMSO).
Ok. I'm assuming then that detergents are compatible with your assay.
Try adding together 1 volume of your DMSO stock with 1 volume of 20% tween-20 (in DiH2O), or any other detergent you have. Use a sonicating waterbath to disperse the drug.
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