My plant extract could dissolve only in DMSO. I tried with different concentrations of DMSO. But it is difficult to dissolve. So can I able to make different concentrations from less concentrated DMSO solution after dissolving in pure DMSO.
First you prepare a stock solution of higher concentration and then you can make different solutions of different concentration depending upon the requirment
Yes you can, prepare a stock of higher concentration and dilute it further as per your need. Keep a negative control for your assay as well
Yes, you can use DMSO for making test solutions. First dissolve your methanolic extract in DMSO to prepare a stock solution and then prepare series of concentration through diluting it in accordance to your experimental need.
You can see this link
For clarification
Maybe you can found what do you want
https://www.researchgate.net/publication/319618700_Determination_of_selenium_in_roasted_beans_coffee_samples_consumed_in_Algeria_by_radiochemical_neutron_activation_analysis_method?_sg=3_PTKjVUhclQj7UcVN2K0KoxElJTr5tSlMermX2_WgePosmjvGw0sbsp02EQkW2Qt8GXxl43cciiRCQBos_jerYSbLuxdEbF46rXNFuH.ILXW3rRBgA3EoAArrlcP2c9Tz1uJZn_KEAEESX1r-hRz9ra6Qn5ENoi6ZXV52_TTbslp3aKxhNXPnOxcIbhChw
Yes you can use DMSO to prepare a stock solution. And use dilution after to prepare your tested concentrations.
use methanol as solvent for antioxidant activity. use methanol to dissolve your extract as well.
Yes, you can use DMSO to dissolve your extracts. You can also make your dilutions in DMSO.
Dmso is used to dissolve the active ingredient. Is it diluted? With distilled water?
DMSO is itself a solvent. It can not be diluted with water. You can make different concentration of extract or compound by adjusting the diluation with DMSO i.e. adding the required amount of DMSO
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