Sumin Mary Jose

11 Questions 13 Answers 0 Followers

Questions related from Sumin Mary Jose

I extracted mucilage from plant. I would like to test its capacity as a binder in tablets, textiles etc. Can anybody give proper suggestions?

I don't know anyone who is expert in this field. I don't have facility to conduct it too. I have done extraction and some characterization on the basis of literature review.

14 May 2019 7,883 2 View

Do Inhibition of protein denaturation consider as a screening test for anti inflammatory activity of plants? If so why ?

Some people give an opinion that Inhibition of protein denaturation is not a relevant work and also cannot perform with plant extract. So like to get new views.

19 March 2019 485 8 View

Hi, which is the proper method for the inhibition of protein (albumin fraction) denaturation by plant extracts?

I am in doubt about different methods of preparations and calculations. While I performed it I got turbid material after denaturation by heating at 57 C for 3 minutes. So the absorbance at 416 nm...

01 September 2018 8,390 1 View

How to dissolve methanolic extract of plant (dissolve only in pure DMSO) in DMSO for antiinflammatory and antioxidant assays?

My plant extract could dissolve only in DMSO. I tried with different concentrations of DMSO. But it is difficult to dissolve. So can I able to make different concentrations from less concentrated...

12 February 2018 2,559 11 View

Can anyone give the reason for getting less value for control in BSA denaturation assay?

I have done repeatedly BSA heat denaturation assay but I get more value for denatured one with sample and test than that of control. I measured in both 660 nm and 416 nm. It is not any coloured...

18 December 2017 9,590 3 View

Can anyone give an idea that whether difference in altitude and climate cause changes in plant's appearance, texture, internodal length etc.?

I have a plant which shows minute differences in different locations. This is having many species with similar properties.

15 November 2017 1,159 5 View

Can anybody give the exact absorbance (wavelength) for inhibition of protein denaturation?

I could see 660 nm and 416 nm in different papers. I have doubt that in this study whether we measure the amount of protein which is not denatured or some other content. If the absorbance gives...

07 November 2017 2,580 4 View

Can any one give the exact calculation to find out the absorbance in albumin denaturation assay?

my value of sample is greater than that of standard and when I consider the formulas such as (1) Percentage inhibition = (Abs Control – Abs Sample) x 100 / Abs control (2) % Inhibition=100 −...

26 October 2017 9,876 6 View

Which is the method for measuring absorbance, turbidity etc?

i confused whether it is colorimetry or uv spectrophotometer?

03 October 2017 8,273 3 View

Can anyone give me a suitable method to dissolve my plant extract for chemical reactions?

Plant is having abundant amount of mucilage. So the extract is resemble to tar. It is difficult to dissolve it for the chemical reactions using different concentrations.I tried with the same...

05 August 2017 1,319 8 View

Anti inflammatory assay: Inhibition of protein denaturation

Do Inhibition of protein denaturation consider as a screening test for anti inflammatory activity of plants? If so why ?

01 January 1970 2,788 3 View