Can anyone help me to remove a hydrophobic peptide from C18 column?

I have injected a hydrophobic peptide into analytical C18 column and i have tried to cleaning the column applying a lot of protocols using acetonitrile, methanol, isopropanol, but in the blank...

05 January 2021 3,305 3 View

Could you help with a doubt regarding UDF velocity inlet profile with multiphase mixture (Ansys Fluent)?

We are two undergraduate students seeking for help!!! We are trying to simulate a laminar flux through a 3D pipe (25 cm long, 2 cm of diameter) consisting of a multiphase flux (air and water). We...

23 November 2020 3,918 3 View

What is the role of Digital Services in Crisis, Disaster, and Emergency Situations?

06 January 2020 7,241 4 View

How come conductivity increased at very low concentrations of CuSO4 after sorption of activated carbon?

I was investigating the sorption of activated carbon on different concentrations of copper(II)sulfate and came across rather unexpected results where the conductivity increased at 0,00001 , 0,0001...

24 November 2018 6,855 3 View

What is a good cycling method for 27F and 519R primers? Tm is 56 and 66, can they be used as a pair?

I'm trying to generate a smaller fragment of 16S from a water sample, so it can be NGS amplicon sequenced. The limit of that service is about 500bp.

14 March 2018 8,032 2 View

Can I use aneuploidy for crossing?

Dear Collegues, we are going to make segregating population of tea plant (camellia sinensis) for genetic research. we need 200 progenies. Can we use aneuploid cultivar as one of the parents to...

19 October 2017 8,451 3 View

Protocol of fungal dna extraction from plant leaf tissue?

Dear collegues, Can we use protocols of DNA extraction from plant tissues (from leaves) to extract DNA of fungal pathogen which lives inside of tissue? If yes, will the quantity of the pathogen's...

11 April 2017 8,448 31 View

If I have low bootstrap values in a ML tree, does it mean that there is no separation between different genera?

I have a maximum likelihood tree (18S and COI) for a nematode family which contains 4 different genera. The bootstrap values were very low and all the 4 genera are intermingled in both trees with...

27 June 2016 593 5 View

Why does the PCR not work occasionally?

Dear collegues! we extracted RNA from apple peel. We need clean RNA without DNA contamination, so we check our RNA samples by PCR. Samples are fresh. and reagents are the same. BUT one time PCR...

24 March 2015 4,855 12 View

Why are there no data displayed in the allelic discrimination plot?

Hello everybody! I'm trying to perform Genotyping experiment using StepOnePlus system. Unfortunately, no data is displayed in the allelic discrimination plot after the run. On the other hand, the...

16 November 2014 1,363 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View