(note: I have tried with 5% Iso propyl alcohol but not satisfactory result)
After transformation how the bacterial own genome can be removed completely from the viable cell? It would be highly appreciated if someone provide information regarding this query.
03 August 2019 2,963 12 View
I am working with Huh7 hepatoma cells. I have to starve the cells for my experimental purposes. If someone would share the experience of cell survival duration without FBS it would be highly...
17 October 2018 623 4 View
How long it takes for complete polymerization of SDS-PAGE resolving (separating) gel? After pouring into the plate it takes one hour for polymerization,is it enough for experiment?
23 May 2016 5,755 18 View
If a mutant cell is cultured in a medium for several batches then what happens in case of the physiology and protein expression level?
08 March 2016 2,451 2 View
respected sir,I am M.Tech student, simulating in matlab on field oriented control (vector control) (d,q compensation) of 3- phase IM using multi-level inverter.In this simulation, direct axis...
16 February 2015 4,781 5 View
What is the suitable optimization technique to find the exact value of Cascaded Multilevel Inverter to apply the field oriented control of induction motor using separate space-vector modulation?
12 August 2014 7,674 4 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
31 July 2024 8,666 3 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
how can I extract my bibliography from researchgate ?
28 July 2024 6,737 1 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View