i am using reference standard grade of amino acids (Reference standard kit, SRL product). i tried dissolving the D-alanine from that kit with ethanol and methanol. but i couldnt able to dissolve it. and i cant use DMSO or acetic acids as it may affect the cytotoxicity of compound. can someone please tell me what is the possible way to dissolve those amino acids? can i use organic solvents like DMF or DCM? if i use will it be cytotoxic?
each and every amino acids have different solubility rate in various solvents. but i dont think it will be dissolved in ethanol. it can be dissolved only by organic solvents like dimethyl formamid (DMF) or DCM. so try dissolving those in less amount of these solvents and see.
http://journals.sagepub.com/doi/abs/10.1177/0269881116652586?journalCode=jopa
Check this link.
Is there any reason why you did not tried aqueous solutions of the amino acids? Potassium phosphate buffer (50 mM, pH 7.4) certainly works...
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