Dear Noureen
1. If you are using CuO NPs For antimicrobial activity, with nanoparticles, they can be dispersed in methanol/ethanol or else using following
CuO nano particles can be dissolved in acids such as HCl, HNO3, H2SO4 and concentrated ammonia solutions. Therefore, you may use a mixtures of phosphate buffer and ammonia. However, firstly, dissolve in ammonia and then dilute with the buffer.
or
Copper nanoparticles are dissolved using DMSO or DMF
or
1.Cu(+II) forms deep blue complexes in ammonia, but you don't need high concentrations of copper to observe them. So the appearence of the deep blue colour does not mean that all CuO was dissolved. It might also depend on the shape (powder or bulk) and on thermal history of your oxide. It is much easier to dissolve when freshly formed than after a long recrystallisation at high temperature.
For ZnO NPs i have work on it and share my experience just simply dissolve in ethanol you wl get the UV-Vis
If you need further assistance about NPs then don't hesitate
GOOD LUCK
Dear Noureen,
Since you are trying to do the UV-Vis absorbance for nanoparticle, as Udayabhanu said, you can't dissolve it in acids because of the material change.
There are two ways to do the absorbance measurement
Hope you get some ideas.
Good Luck!!!
Please have a look at link below
http://iopscience.iop.org/article/10.1088/0957-4484/27/32/324001/meta
@ Udayabanu, I read the answer which you suggested and that was a good answer. I was about to say as you said it's not a suitable method to dissolve in acids because it's changed into another form. But unfortunately in a hurry I typed in another form which mislead with another meaning. Sorry for the typos. I have edited the answer now.
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