Is there any chemical methods to treat etbr gels , so as to precipitate etbr or negate its harmful effect
Not that I know. In our university EtBr-containing gels are going into a separate waste. A guy comes and picks the stuff up. No clue how he is disposing of them but I guess there will be special regulations for toxic waste ...
Dear Himanshi,
You can do the following
Buffer Solutions containing very small concentrations of ethidium bromide (less than 0.01% by wt.) may be discharged to sinks
Agarose Gels containing trace amounts of ethidium bromide (0.3-0.5 μg/ml) may be disposed of daily in double bag-lined containers (e.g., five-gallon pails).
Stock Solutions, Crystals and Powders typically contain relatively high concentrations of pure ethidium bromide. Place empty stock solution bottles or unused stock solution bottles in double ziplock plastic bags. Place absorbents into bag if available.
Keep waste containers in secondary containment bins, closed when not in use, and labeled as "Hazardous Waste, Ethidium Bromide (Mutagen)". When labeling, be sure to check the "Other" box under hazard type and specify "Ethidium Bromide (Mutagen)". Store waste containers at or near hazardous waste satellite accumulation area.
Spill Cleanup-Contaminated Debris
Includes ethidium bromide contaminated debris (e.g., gloves, mats, tips, spill clean-up waste) from spills of Stock Solution, Crystals or Powder. Collect waste in double bags. Bags must remain closed when not in use and labeled as "Hazardous Waste, Ethidium Bromide (Mutagen)". When labeling, be sure to check the "Other" box under hazard type and specify "Ethidium Bromide (Mutagen)". Store waste bags at or near hazardous waste satellite accumulation area.
Best Regards
http://www.ncku.edu.tw/biochem/E-S-H/SMOU-Cleanup_of_Ethidium_Bromide.pdf
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