Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Best rat model of Type 2 diabetes and metabolic syndrome?

Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you

04 September 2020 3,661 4 View

How can I digest the brain tissue in healthy mice as evaluating the apoptosis using flow cytometery by annexin V/propidium iodide (PI)?

We digested the brain tissue with Trypsin ( 1x) for 15 min (all steps were performed in cold conditions) and suspend the cells by pipetting up and down (3-4 times) to the single-cell suspension....

22 August 2020 5,611 4 View

Hi, is there anyone tell me or share me a link for downloading the template for "Cities" journal of Elsevier?

I went through the cities journal website but unluckily couldn't find the template

28 June 2020 811 3 View

Can anyone suggest a kit or protocol to extract Brucella spp. DNA from FFPE blocks?

Hi everyone! I am trying to extract Brucella DNA from FFPE blocks. Can anyone suggest me any good kit or protocol? I checked many articles and kits but still could not find an appropriate kit or...

15 December 2019 9,767 2 View

Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ?

Hello, Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ? Thank you

06 April 2019 8,782 19 View

What is the contribution of renewable energies in the fight against global warming?

The phenomenon of the greenhouse effect is a threat to the environment. What is the contribution of renewable energies in the fight against global warming?

04 April 2019 1,239 33 View

Methodology for implementing a thesis ?

Methodology for implementing a thesis involves: - State of the art of the studied phenomenon - Study context - Introduction - Hypotheses - Objectives - Labor interest - Methodology apprehended -...

14 February 2019 6,502 16 View

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

24 January 2019 5,584 2 View

How is 3D pelvic kinematics examined during walking before and after interventional exercises in patients with pelvic inclination?

in general, for healthy people, in the 3D motion analysis to define the anatomical coordinate system on segments, one frame in static position is taken by the camera, which will make segment...

10 October 2018 4,098 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Are there SDMs that can take in percentage data?

Hi everyone, I am wondering if anyone knows of a species distribution model that can take in percentage data (i.e. presence data with prevalence values attached to them)? The dataset I have...

18 February 2021 7,312 3 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

Why are my Maxent SDM projections coming up almost entirely blue?

Hello Maxent Community, I have been generating SDM models and projections in Maxent. I have had some success with one set of variables (some Hydroshed variables mixed with World CLIM variables);...

19 January 2021 3,994 7 View