Hi everyone,
I need to prepare ngm plates supplemented with fatty acids to grow C. elegans. Thus, I would like to know which solvent can I use to dilute fatty acids (myristic acid and linoleic acid) to make a stock solution?
Thank you
Hi Vitor,
In the NGM recipe (http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html), ethanol is used to dissolve cholesterol, therefore it can be used to reconstitute both fatty acid solutions.
It is based on your processing conditions. However, hexane is most frequently used to dilute fatty acids while its quantification.
I think you may want to take a look at this paper.
Article Dietary Supplementation of Polyunsaturated Fatty Acids in Ca...
Probably, we can use hot DW to dissolve those fatty acids. Make sure you use NP40 (0.1%) to enhance the FA distribution and absorption. In my experience, NP40 works well in both agar and lipid culture. Hope it can help you.
Hi
The processing protocols are well explained in the following paper:
https://doi.org/10.1007/s12161-021-02203-0
I hope this helps!
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