In the literature I found this: "after 3 day post-confluency, cells are differentiated." But how? With or without FBS? If with, than 1, 5, 10% or changing? How to prevent outgrowing? Thanks
Magdolna: It's hard to understand "differentiated" out of the context for HK2 cells, which are highly differentiated cells with unusual functions. These polar cells form a single layer and tight junctions, and are good model for studying polar secretions. We found them easy to grow and needs minimal FBS- they make plasma proteins in a fashion resembling hepatocytes (see our paper attached).
Dear Ke-Wei Zhao! Thank you for the answer, and the attached paper. You are right, I was not clear in my question. My aim is to see cilia, and then I would like to do immunostaining to examine protein localization. Cilia can not be detected during proliferation, so I would like to stop proliferating and wait for cilia appearence. That is what I ment in "differentiation".
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