If I'm right, dopaminergic neurons in substantia nigra but not in ventral tegmental area are affected by Parkinson's disease. I have stained brain slices with TH to observe changes in dopaminergic neurons in Parkinson's mouse model. In the confocal images obtained after staining, I could not demarcate dopaminergic neurons belonging to the substantia nigra and ventral tegmental area. I'll appreciate it if you could suggest to me the way to find the TH-labeled neurons affected in Parkinson's diseases. I have attached images I obtained and I use ImageJ for TH positive cell counting.
Thank you
Saroj
Hello,
according to a paper from Thompson et al. in mice (!) you can perform double stainings for TH and Girk2 to determine dopaminergic neurons from the Snpc from VTA (Thompson L, Barraud P et al. (2005) J Neurosci, 25: 6567-6477).
I do not know if this works in rats.
Otherwise you can use the Atlas "Rat brain in sterotaxic coordinates" from Paxinos (or the Mouse Atlas for mice). This can help you to delineate the Snpc from VTA.
Kind regrads
Stefan
Keep in mind that the SN and VTA are more or less arbitrarily designated as distinct areas--more so in mice brains.
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