Saroj Kumar Sah

9 Questions 13 Answers 0 Followers

Questions related from Saroj Kumar Sah

When can we use Fisher's Least Significant Difference (LSD) test?

Hi, Following a two-way analysis of variance (ANOVA), when can we use Fisher's Least Significant Difference (LSD) test? Is it reliable or explainable showing data? I have some mouse behavior data...

29 September 2020 9,595 5 View

For how many days do mice remember Morris water maze test tasks?

Dear all We have conducted Morris water maze test using mice of the neurodegenerative disease models. We want to repeat the test using the same mice. I'm wondering whether mice keep the memory of...

19 February 2020 1,236 3 View

How to distinguish axon and dendrites and quantify tau/p tau in neuron by imageJ?

Hi, I want to quantify total tau, phospho tau and tubulin in the axon and dendrite of hippocampal and cortical neurons by Confocal microscopy analysis. I'll appreciate if you could suggest me the...

11 May 2018 1,483 5 View

Would you please recommend some phospho-Tau antibodies for western blot analysis using human brain tissues?

Hi, Would you please recommend some phospho and tau antibodies for western blot analysis that best suit for human brain tissues? I had done my experiment with tau antibodies (Abcam) a couple of...

09 March 2018 7,095 6 View

Ageing phenomenon and memory impairment in 3xTg Alzheimer's disease mice?

Dear Sir/ Madam, We had conducted a study on memory impairment process in 3xTg AD (3xTg Alzheimer's disease) mice model. We observed significant memory impairment in these mice at their age of 24...

23 March 2017 4,853 5 View

Does re-probing for primary antibody with different origin need stripping for western blot PVDF membrane?

Hi, Do we need stripping for western blot membrane(PVDF) when we have to re-probe with a primary antibody having different origin in comparison to the previous one. Previously I had probed with...

11 April 2016 7,769 4 View

Western blot electrophoresis insufficient, no migration of protein sample and prestained protein marker ladder below 35 kDa.

I am getting a problem doing sds page gel electrophoresis of my protein sample(mice cortex lysate). When I do electrophoresis with 12% sds page gel, the protein marker ladder do not migrate below...

23 April 2015 8,229 10 View

How to differentiate dopaminergic neurons in between substantia nigra and ventral tegmental area in TH labeled mouse brain slices?

If I'm right, dopaminergic neurons in substantia nigra but not in ventral tegmental area are affected by Parkinson's disease. I have stained brain slices with TH to observe changes in dopaminergic...

01 January 1970 6,367 3 View

How to differentiate dopaminergic neurons in between substantia nigra and ventral tegmental area in TH labeled mouse brain slices?

I have stained brain slices with TH to observe changes in dopaminergic neurons in Parkinson's mouse model. In the confocal images obtained after staining, I could not demarcate dopaminergic...

01 January 1970 3,282 1 View