How to differentiate dopaminergic neurons in between substantia nigra and ventral tegmental area in TH labeled mouse brain slices?

01 January 1970 1 3K Report

I have stained brain slices with TH to observe changes in dopaminergic neurons in Parkinson's mouse model. In the confocal images obtained after staining, I could not demarcate dopaminergic neurons belonging to the substantia nigra and ventral tegmental area. I'll appreciate it if you could suggest to me the way to find the TH-labeled neurons affected in Parkinson's diseases. I have attached images I obtained and I use ImageJ for TH positive cell counting.

Thank you

Saroj

Alpana Singh

Hi,

I am just providing a vague idea, the VTA lies in the centre of midbrain ( in your images, the rightmost border) while SNpc lies in the branch coming out of it. In PD, predominantly SNpc neurons ate affected. You can read one of my article from 2016 (which is available on research gate) wherein I have used MPTP induced PD model in mice.

Thanks,

Alpana

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