Hi all,
I would like to differentiate granulocytes from murine bone marrow cells. Does anybody have a protocol?
Thanks in advance
Hi Kevin,
this might help:
http://www.jbmethods.org/jbm/article/view/12/6
All the best !
Hi Swarnendra,
thank you for your answer, but I already culture dendritic cells and macrophages from bone marrows. I need a specific protocol for granulocytes (i.e. days of culture and amount of cytokines), since I can't find it anywhere...
Dear Kevin,
What kind of granulocytes are you trying to culture: Eosinophils, basophils, or neutrophils? Or maybe all of them at once?
Joakim
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