How to differentiate bone marrow-derived granulocytes from murine bone marrow?

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Swarnendra Singh

Hi Kevin,

this might help:

http://www.jbmethods.org/jbm/article/view/12/6

All the best !

Kevin Leone

Hi Swarnendra,

thank you for your answer, but I already culture dendritic cells and macrophages from bone marrows. I need a specific protocol for granulocytes (i.e. days of culture and amount of cytokines), since I can't find it anywhere...

Joakim Dahlin

Dear Kevin,

What kind of granulocytes are you trying to culture: Eosinophils, basophils, or neutrophils? Or maybe all of them at once?

Joakim