SCAR markers are used to track bacterial population in the rhizosphere. Can you help in providing protocols for developing SCAR markers, tagging SCAR markers with pseudomonas strain and monitoring its fate in the rhizosphere?
Can any tell me step by step instructions for developing SCAR (Sequence characterized amplified regions) markers for Pseudomonas fluorescence. Also can you help me how to monitor or track pseudomonas population in rhizosphere using SCAR markers?
Sakshi, you can follow these reports:
Development of a strain-specific quantitative method for monitoring Pseudomonas fluorescens EPS62e, a novel biocontrol agent of fire blight http://www.ncbi.nlm.nih.gov/pubmed/16006071
Quantification of Pseudomonas fluorescens strains F113, CHA0 and Pf153 in the rhizosphere of maize by strain-specific real-time PCR unaffected by the variability of DNA extraction efficiency http://www.ncbi.nlm.nih.gov/pubmed/20153383
Direct and Specific Assessment of Colonisation of Wheat Rhizoplane by Pseudomonas fluorescens Pf2 http://link.springer.com/article/10.1023%2FA%3A1022084728087
Regards
Alex
Please read our recent paper published on this, which will tell all about SCAR marker
Balachandar
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