Hello everyone,
I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA.
My first question is,
I wanted to develop the MCF7 mouse xenografts. I did a search and came to know that the injection of estradiol is recommended to develop tumours prior to injecting the MCF7 cells. Also, I have seen some papers without estradiol. Can you tell me the role of estradiol in tumour formation? Can the mice form tumour without estradiol?
Lastly,
Once the tumour reaches a definite volume, I wanted to inject my compound of interest. Here is the question, which is a simple way to inject? I have found the following ways related to my studies.
1. Trochar
2. Microosmotic pump
3. Intraperitoneal
4. Oral gavage
Please recommend me a simple way to introduce my compound to the mice. If you have a better option also welcome.
Many thanks in advance,
Arun.
Hi Arun,
In our experience we always use estradiol pellets with MCF7 xenografts. This is an ER positive cell line and it requires extradiol for growth. We use the pellets from Innovative Research of America and place them with a trochar (IRA has the info on the trochar) 3 days before implanting cells. We usually use between 10-20 x 10^6 cells per mouse for flank implantation.
http://www.innovrsrch.com/
For delivering a drug it depends on which drug you will be giving your mice, if you know the bioavailability, that will determine if it's better to give it orally, IP, SC, etc. Ideally if you are testing a new drug you would like it to be orally bioavailable because it's the easiest way for a patient to take it.
Here are a couple of our publications that explain the xenograft model with MCF7:
Article .Antiestrogens in combination with immune checkpoint inhibit...
https://www.nature.com/articles/1204729
Let me know if you have more questions,
Diana
Hi, Following the same line of previous answers, I'm assuming that you compound will need a vehicle or solvent, here I'm attaching a PDF file that can be usefully decide the route at least in terms of the nonclinical used vehicle and different routes. Best,
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