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Hello everyone, Can someone help me with this? My colleague initially used the EndNote citation manager to insert the citations in our manuscript. The manuscript has now been transferred to me...
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Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...
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Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...
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Hello, everyone. I'm trying to subclone a 5 kb gene to a vector (approx. 5 kb). Initially, I designed the primers which have restriction enzymes overhang (XhoI and XbaI). I amplified my gene using...
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