Which is best tool for alignment of large bacterial sequence?

Hi! I need to align big fasta files (bacterial 16S) . I have tried to align them in some web-servers and freeware, but my files are too big to be run in there. I will be very glad if you could...

16 January 2021 1,266 4 View

What is the best studying timeline after injection of B16-F10 melanoma metastases cell line?

I want to inject B16F10 cells into C57BL/6 mice as a melanoma metastases model to study the speed of metastases in different organs. I need advice about the designing of the best timeline for...

14 January 2021 3,704 1 View

Which type of press is proper and common for making PZT parts?

What kind of press is using to make electroceramic parts, including PZT parts (hydraulic press, pneumatic press, etc.)? Furthermore, what is the reason for using this type of press? And does the...

28 December 2020 1,215 2 View

No smears on gel after cDNA synthesis ?

Hi all, I have a problem with detection of cDNA synthesis where when I put it on the gel , there were any smears so what the meaning of that and what can I do? Thanks

21 December 2020 1,126 2 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

How to dye DOPC liposome with Dio c18 fluorescent dye?

13 November 2020 9,244 3 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

Why 3.5% wt NaCl solution has been considered to simulate sea water in corrosion tests?

As you know, Test electrolyte in most of the academic corrosion studies have been considered as 3.5% wt NaCl (Rerfer to Standard ASTM G44) and in other standards like NACE 1D182 the concentration...

09 October 2020 8,625 17 View

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange?

The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...

10 May 2020 7,879 3 View

Are ACEIs underdeveloped in children?

COVID19 shows less affection of lung in children . this may be due to underdeveloped or decreased expression of ACEI in lungs of children taking in consideration that as age increase from 5...

28 March 2020 2,448 2 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View