@SRR8393705.1 1 length=253
TACGGAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCGGGTAGGCGGCTAGACAAGTTGGATGTGAAAGCCCTGGGCTCAACCCAGGAAGTGCATCCAAAACTGTCCGGCTTGAATCTCGGAGGGGGACAGGGAATTCCCGGTGTAGAGGTGAAATTCGTAGATATCGGGAGGAACACCAGTGGCGAAGGCGCTGTCCTGGACGATGATTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGN
+SRR8393705.1 1 length=253
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Will this be single end or paired end as no identifier #0/1 or /2 are given here. Also the metadata on SRA run selector gives it is single end but in the research article it is said that the study is paired end (2*300bp)
Additionally, when I checked the plotQualityprofile using R it was not quite good and total no. of cycles given were 500.