Jorge Enrique Vazquez Buchelli

2 Questions 1 Answers 0 Followers

Questions related from Jorge Enrique Vazquez Buchelli

Why do I have a Jagged line on my qPCR amplification?

I am performing a qPCR standardization of some MGB probes. I have tried just a few times but I don't want to waste reagents, every time I tried the assay, there is a jagging line. I am thinking...

14 November 2019 4,676 3 View

Can a probe work with less Tm than the primers?

I found 2 interesting articles for the detection of bacteria at Phylum level with MGB probes, claiming that they work. I want to use that primers however when I use the oligoanalyzer tool, it...

09 July 2019 6,494 3 View