According the literature and the your proposed experimental work according the desired properties.

I agree with Dr. S. Kalidhasan.

1. Generally, you should take same amount as standard (similar nanoparticles used in previous studies; nanoparticles with known values in such experiments) and compare your nanoparticles with it.

2. In drug delivery, you will need to take amount of nanoparticles that contain specific dose of drug. For example, you may need to take 3 grams of nanoparticle A and 4.4 grams of nanoparticle B for dose of drug equivalent to 500 mg.

3. In some cases, limits are also put by experiment/equipment on minimum and maximum amount of nanoparticles to be used.

You should explain more about type of your nanoparticles and experiment to acquire more meaningful ansswers. Best regards.

Hi,

Please elaborate your question. Which experiment?

If the question is about to know the concentration of the nanoparticle sol to be used in the experiment, then the answer can be as follows

1. By knowing the nanoparticle precursor concentration in the initial solution and calculating the volume change during the synthetic process one can know the concentration of the nanoparticle in the final solution

2. If the nanoparticle sol is UV-Vis active and one is unaware of the concentration of a given nanoparticle one need to first prepare a set of solution of the same nanoparticles with different concentration and then by colorimetric method it is possible to find out the concentration of the nanoparticle solution.

3. Yes AAS,ICP-AES and EDAX can also be used for determination of the amount of the nanoparticles. 

I agree with Dr. S. Kalidhasan and Mubashar Rehman.

Thankyou all for the response. 

We are focusing on the delivery of different (gold, silver, magnetite etc) nanoparticles into live mammalian cells. 

I agree for clarification of the question but You can also use the  ICP-AES (Inductively coupled plasma atomic emission spectroscopy)  for the solve your problem. it is technique used for detection of trace metals. The emission of electromagnetic radiation at particular wavelengths is characteristics of particular  element that is produced by the excited atoms or ions with intraction of Inductively coupled plasma (flame techniques) temperature range from 6000 to 10000 K.  It is also useful for  solution chemistry & standard silicate dissolution methods are used. The intensity of this emission is indicative of the concentration of the element within the sample. but the high temperature range damaged the mammalian cells. So it need to cure of live mammalian cells during the determination of concentration of nanoparticles. I hope the answer is helpful for your work.

You will need to calculate number of cells and nanoparticles because efficacy (and toxicity) of nanoparticles is influenced by number of nanoparticles internalized. Please see following for guidance:

1.         Soenen SJ, De Cuyper M. Assessing iron oxide nanoparticle toxicity in vitro: current status and future prospects. Nanomedicine. 2010;5(8):1261-75.

2.         Soenen SJ, Rivera-Gil P, Montenegro J-M, Parak WJ, De Smedt SC, Braeckmans K. Cellular toxicity of inorganic nanoparticles: common aspects and guidelines for improved nanotoxicity evaluation. Nano Today. 2011;6(5):446-65.

If you use nanometal  atomic absorption spectroscopy is very good.  The color of  your  samples in low concetration is an other method. Icp ms is other methods. More over during the  experiment  SPR sensor is suitable for mesure the concentration.

At the first let us know what kind of experiment. Second: let us know what kind of nanoparticles and what kind of matrix - if matrix will be.