I'm interested in the spectrophotometric determination of chloropyriphos pesticide.
Gold nanoparticles (GNPs) play an imperative role in chemistry and biology by virtue of a large surface-to-volume ratio, quantum confinement, and other unique properties. Gold nanoparticles consist of several kinds of interesting properties. GNPs, with the diameter of 1-100 nm, have high surface energy and high surface-to-volume ratio to endow with a steady control of a large amount of biomolecules retaining their bioactivity. Moreover, GNPs have a capability to consent fast and direct electron transfer between a wide range of electro active species and electrode materials. Gold
nanoparticles may potentially be another useful material for removing contaminants, such as toxic chlorinated organic compounds, pesticides and inorganic mercury, from water.
Different concentrations of Chlorpyrifos pesticide solutions can prepared freshly using ethanol solvent. Then these solutions will be diluted with water to make a final concentration of 2ml. 2 ml of Gold Nanoparticles solution will be added to these pesticide solutions and mixed for five minutes. Finally 1 ml of 10 M NaCl solution will be added and refluxed for 10 min.
Perkin-Elmer model Ultraviolet-Visible Spectrophotomer can be used for spectral analysis. Scanning Electron Microscopic (SEM) image can be taken using a FEI QUANTA-200 SEM instrument. High Resolution Transmission Electron Microscopy (HRTEM) can also be carried out using a 300 kV JEOL- 3011 instrument with a ultra high resolution (UHR) pole piece.
Dear Mr. Raj,
Chlorpyrifos is an organic molecule that have conjugate systems in their structure. in general, chemically conjugated systems absorb UV in the range of 200‐380 nm. One benefit of this method is its easy handling and non-destructiveness.Thus, you may use UV method for the detection and quantification of chlorpyrifos. As per my suggestion, the better way to analyse your sample is first extract chlorpyrifos from water using isoctane or hexane and then make known concentration of your sample in acetonitrile and run UV @ presetted absorption range of 190-400 nm. you may standerdised and validate this method using authentic/standard chlorpyrifos in known concentration.
I think following links will be more helpful in this regard.
http://onlinelibrary.wiley.com/doi/10.1002/jhrc.1240180312/pdf
https://elibrary.asabe.org/abstract.asp?aid=29191
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