What pH buffer solution best replicates physiological conditions?

I am studying the interaction between protein (HSA) and liposomes (made with DMPC) and I would like to study under physiological pH for application in studying protein interaction with DDS. I have...

10 November 2020 3,594 3 View

All teacher educators are under the lockdown since March, what could they be doing, how is this lockdown going to affect tutors' performance ?

There are many COVID 19 related issues in terms of how to deal with the continuum of teaching and learning. I am meditating about what the future performance results will be for both the student...

16 June 2020 199 3 View

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03 December 2019 5,253 1 View

Reliable conjugated and unconjugated IL17-F anti-human antibody for flow cytometry?

Dear all, can someone kindly recommend a reliable fluorochrome-conjugated IL17-F anti-human antibody and also an unconjugated one please? I am studying CD3+ Lymphocytes from healthy volunteer...

19 February 2019 3,843 3 View

What is an acceptable sampling rate for skin conductance?

What is an acceptable sampling rate for measuring skin conductance? Research seems to use various rates, from 32Hz to 1000Hz. Is there a minimum rate advised? I have a device that measures at 4Hz...

03 February 2019 9,630 1 View

Is it possible to prepare SDS Reducing Buffer without including the bromophenol blue?

Hi all, I am doing an SDS-PAGE (Laemmli) gel electrophoresis but don't have any bromophenol blue to make up my SDS sample buffer. Is it possible to leave this out if you are confident of how...

26 January 2018 2,096 3 View

How to calculate the Feed Conversion Rate (FCR) in free-choice diets?

Hi! I would like to calculate the FCR for a diet-induced obesity experiment in rats. Since I have many nutrients/groups, I am calculating the food intake (FI) in kcal, not in grams of food. Has...

09 November 2017 5,258 5 View

What is the typical percentage error when corneal endothelial cells are counted using a specular microscopy ?

11 July 2016 7,906 2 View

How do I design Methylight primer/probes without using Beacon?

I want to Methylight assays and I need to be able to design the primer/probe sets, or get software that can do it for me. But I've only been able to find Beacon software that does both primers and...

08 June 2016 9,414 1 View

What Australian cities would be good to compare with Geelong, Australia in regard to economic, social & physical capital?

Newcastle and Wollongong are good examples but I am interested to learn about other large regional centres, particularly those that may have experienced a shift in their economy and have had to...

26 November 2015 5,759 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

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03 March 2021 5,360 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View