Is there any difference betwwen Z-average (d.nm) calculated by instrument itself and calculation of average by number particle size distribution?

Generally using Malvern zeta sizer gives particle size distribution as Z-average in nanometer (nm) but if particle size distribution may be calculated using Number stats table using formula given...

02 March 2019 3,458 6 View

What should be the basis of selection of excitation maixima to get emission maxima of a fluorescent molecule ?

I have prepared carbon dots (CD) using plant material and got its absorbance maxima at 231 nm through UV-Vis spectrophotometer. Then what should be excitation maxima of Carbon dots during its...

10 November 2018 7,683 7 View

Whether fluorescence of any molecule may change in the presence of any buffer?

Actually i have prepared carbon dots using lactose and checked its fluorescence by preparing carbon dots solution in distilled water. then, another carbon dots solution was prepared in distilled...

09 October 2018 8,671 3 View

Why nanoparticles are considered better than their corresponding ions?

I want to understand the concept of nanoparticles. nanoparticles are considered better due to their size but as nanoparticles are prepared from corresponding ions by taking up electron through...

06 July 2018 9,125 16 View

How to find the particular protein domain using bioinformatics tool?

I am looking to find thioesterase (Te) domain from tyrocidine synthetase C (TycC)from Bacillus brevis ATCC 8185. I can able to find the TycC domain in ncbi but unable to find the 756 bp Te domain.

10 November 2017 9,191 3 View

Is there a difference between the turbidimetric and the well-diffusion method for MIC determination?

I am determining antibacterial activity of isoniazid derivative against bacterial strains (mycobacterium smegmatis, E. coli & S. aureus) by turbidimetric method and I got good inhibition but...

02 March 2015 5,023 5 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View