Technique of Plant analysis to resolve iron Chlorosis, Ferrous (Fe+2) and Ferric (Fe+3) from Soil and Plant
There are several methods using HCl and orthrophenanthroline and Direct through AAS and all have their limitation. we took this estimation seriously worked for nearly 5 years and found the best way was its estimation and speciation through Ion chromatograph which we published in Communication in soil science and plant analysis during 2014 as per the link below.
- Himanshu Bariya 📷Amrit Lal Singh and 📷Vidya Chaudhari 2014. Measurement of Fe(II) and Fe(III) in Groundnut by In-column and Post-column Reactions in Ion Chromatography .Communications in Soil Science and Plant Analysis 46(3):1-9 DOI: 10.1080/00103624.2014.981272
When the Fe deficiency-induced changes were compared with the response to salt stress, it was found that the vast majority of the transcriptome is altered by environmental stress, and that these changes are most dramatic in the root epidermis. Interestingly, there is also a small set of genes unaffected by stress; this core may define the essential features of each cell type, and mediate the appropriate transcriptional responses to environmental stresses. Of the changes in the epidermis, two specific strategies of Fe uptake have been identified in plants. Non-graminaceous plants reduce Fe3+ via a membrane-bound reductase to make it accessible for uptake by a Fe2+ transporter, while grasses secrete phytosiderophores (PS) that readily bind Fe3+, and the Fe-PS complexes are then transported back into the roots.
The NADPH-dependent ferric chelate reductase, AtFRO2, then reduces Fe3+ to Fe2+. Electrons are transferred from NADH+ across four heme groups, to Fe in the rhizosphere. This appears to be the rate-limiting step in Fe uptake in Arabidopsis. In fact, the transgenic overexpression of ferric chelate reductases in the roots of rice, tobacco and soybeans has been successful in increasing tolerance to Fe-limiting conditions.
Once reduced, Fe (II) can then be transported into the root epidermal cells by the divalent metal transporter AtIRT1 AtIRT1 also transports Zn, Mn, Cd, Co and Ni. Additional root epidermal transporters for these metals have not yet been identified; but in the irt1-1 loss of function mutant, shoot accumulation of Fe, Mn, Zn, and Co decreases significantly, and the plants become Cd tolerant,20, 25suggesting that AtIRT1 is a primary transporter for these metals under Fe deficiency. A similarly broad range of metals was found to be transported by the tomato orthologs LeIRT1 and LeIRT2, which complement yeast mutants defective in the uptake of Fe, Zn, Mn, and Cu. Thus, the Fe deficiency response also leads to the uptake of metals other than Fe, all of which are potentially toxic.
Spectrophotometric determination of Fe2+ ions using
1,10-phenanthroline
Measurement of Fe(II) and Fe(III) in Groundnut by
In-column and Post-column Reactions in Ion
Chromatography
A simple spectrophotometric method for the
determination of iron(II) aqueous solutions
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology
- Enzymes