How to determine cut off cq value in qPCR?

15 February 2023 1 1K Report

How to determine cut off cq value -qPCR assay in non-predefined mothed? is it related to total number of cycles used? specially in low copy targets.

Héctor ALAN HERNÁNDEZ Pérez

Hola Abinet, te comento que hace unos años vi una información relacionada con lo que preguntas, espero le sea de utilidad.

Alba-Aguayo y Rueda A. (2013). Problema Bioquímico Determinación del ciclo umbral y la eficiencia para la PCR cuantitativa en tiempo real. Rev Educ Bioquimica.;32(1):36-39.

Article Respuestas al problema bioquímico: Determinación del ciclo u...

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