Fluorescent tyramide protocol ?

Hello, Im asking to check if anyone has a protocol for preparing fluorescent tyramide for fluorescent in situ hybridization. The one Im using isnt working, so if anyone has one that works, can you...

05 November 2020 9,566 3 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

Unbalanced data in linear and nonlinear mixed model ?

when fitting a nlme on nested repeated measurements data , models can be unstable if sample sizes across groups are highly unbalanced. In my dataset I have 4 unbalanced groups in terms of...

24 June 2019 1,698 4 View

Is soxhlet extraction a reliable way of getting pure THC from cannabis plant?

I have been having isssues getting pure THC form the USA and it seems the price from sigma is exhorbitant. I want to get the plant and extract THC. i was told by someone that soxhlet extraction...

15 August 2018 8,300 9 View

To identify clinical isolates of S. aureus and P. aeruginosa to strains level using Sanger Sequencing, which primers shall I use for each species?

I want to identify the clinical isolates of S. aureus and P. aeruginosa (identified to species level using MALDI TOF) to strains level. I am planning to use Sanger Sequencing. But I am not sure...

24 June 2018 7,126 3 View

How can i adjust parameters in logistic regression?

26 February 2016 1,440 13 View

Is anyone interested in collaboration on a study of the self-replication and epidemic aspects of a marketing strategy?

I am trying to compare an online/offline self-replicating marketing strategy to Von Neumann and Biological methods at the first stage and then to an SIR epidemic model at the second stage. The...

25 March 2014 6,377 1 View

Can anyone brief me about research design effects?

I remember that I have learned a calculated sample size will be multiplied by some number if a multistage sampling is used. I want to have further information on this: is it applicable to all...

12 February 2014 5,988 1 View

scope of biotechnology

hai can any one help me ,,,...am 2nd year biotech student....am intrested in genetics...so i selected my elective "animal and plant biotechnology""...can any one say is there any scope for...

17 April 2011 9,039 1 View

Could not load dynamic library 'libcusolver.so.10'; dlerror: libcusolver.so.10: cannot open shared object file

System information OS Platform and Distribution (e.g., Linux Ubuntu 18.04): Ubuntu 20.04 Python version: 3.6 Installed using virtualenv CUDA/cuDNN version: 11.5 / 8.1.0.77 GPU model and memory:...

01 January 1970 9,545 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View