I am planning to evaluate the efficacy of certain phages on planktonic and biofilm forms of S. aureus using colony count. Could any one advise me what chemical to use to stop the effect of phages (kill phages) on the colony count media without affecting S. aureus ?
Thank you in advance,
Legesse
Dear Legesse,
If you can sufficiently dilute your cell - phage sample, it should be possible to see colony growth on plates and capture both total resistant and sensitive cell numbers. If you are really worried, it might be possible to filter cell - phage mixtures and retain and plate the cells.
Regards, Andrew
I don't think there will be any good way to selectively kill the bacteriophages. However if the extracellular phage titer is not that high in the media then you might be ok to get a cell count upon serial dilution, as Andrew has pointed out. He suggests you might filter out the cells to discard the culture supernatant or you could also just collect cells by centrifugation. Neither method will completely get rid of the phages but might dilute them out sufficiently that when you make your serial dilutions to count cells most won't be infected.
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