I am working with samples that are approximately 10-12.5 mL after preparation of vesicles ranging in size from 400 nm to 6 um. I believe I have different structures being formulated that is giving me such a broad size range. What chemical method could be done to determine what species are left as I filter the particle down (6 um filter to 1 um filter etc...) I need to really understand the particle and I feel a chemical test may be beneficial, but I do not know of any that can be done.
In my opinion (if I understand your question correctly), it depends on what kind of substances you have in the whole liposomal suspension. For the trace of phosphatidylcholine, I used Bartlett assay for determination (which quite sensitive to contaminated inorganic phosphate). Therefore, if you want to use this method you have to ensure that you dont have any other trace of phosphate in your sample except phosphatidylcholine.
Concerning to the structure and size of liposome population: I don't know how is your method of liposome preparation: if you don't e.g extrude (pass the liposomes through a membrane, not through a filter) the initial population, you should have a broad range particle size and type of liposomes (multilamellar, oligo-, unilamellar). Extrusion downsized and homogenize the size (and also the structure) of the population; filtration may remove part of the lipids from the filtrated solution.
You can do a TEM scan of samples to see the species. If more uniform distribution is desired you may consider extruding the liposomes through series of membrane filters.
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