I am trying to image liposomes in a confocal and EPI fluoroscence wide-field microscope. I have labeled two species of interest and when I tried to image them and capture images, I had difficulties with both microscopes due to flow/movement of the liposomes.
Is there a staining material that I could use that would work imaging vesicles or is there a way to image them in place without one?
Liposomes and substrates can be considered for chemical modification to allow liposomes to be adsorbed to the substrate by chemical bonds; it is also contemplated to adsorb the liposomes to the substrate by electrostatic forces between the liposomes and the substrate. If you are worried about multi-layer stacking, you can dilute before smearing.
If you can, use a fast camera, provided your fluorophore allows that. You can try and slow the flow by making the medium a bit viscous in possible, but maintain the osmolarity of course your liposomes don't collapse. I had similar problem when taking images of GUVs in a recent paper, but increasing the frame speed helped circumvent the issue (https://www.nature.com/articles/ncomms14867). See Figure 7.
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