Hi everyone, Can somone help with protocol of total phosphorus content in olive pomaces ? Thanks.
There are various methods available for determining the total phosphorus content in olive pomaces. One commonly used method is the acid digestion method. Here are the steps:
1. Weigh a representative sample of olive pomaces (about 1 gram) and transfer it to a digestion flask.
2. Add 5 mL of concentrated nitric acid to the flask and swirl to mix.
3. Place the flask on a hot plate and heat it gradually until the mixture boils. Keep boiling for about 30 minutes.
4. Allow the flask to cool to room temperature and then add 5 mL of concentrated perchloric acid to the mixture. Swirl to mix.
5. Heat the flask again until the mixture boils and keep boiling for about 30 minutes.
6. Allow the flask to cool to room temperature and then add about 50 mL of distilled water to the mixture. Swirl to mix.
7. Transfer the mixture to a volumetric flask and make up the volume to 100 mL with distilled water.
8. Analyze the solution for phosphorus content using a suitable method, such as colorimetry or ICP-OES.
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