Compost processed from urban wood and leaves could act as a transmission vector of phytopathogens when recycled into gardens and city parks. There are many studies that analyze composition by sampling the compost and then using cultures to finally extract DNA to identify fungi. Maybe direct DNA extraction from compost (NGS or Real Time Sequencing) could be used to quick sample identification.
Thank you
Pablo Pérez Daniëls It is usual way. We always have got
Botryosphaeriaceae, Nectriaceae and other plant pathogenic fungi in routine NGS analysis and our final task - to choose indicator species for soil health evaluation by real-rime PCR.
See some publications for the methods
Belair M, Pensec F, Jany JL, Le Floch G, Picot A. Profiling walnut fungal pathobiome associated with walnut dieback using community-targeted DNA metabarcoding. Plants. 2023 Jun 20;12(12):2383.Article Diversity of Endophytic Fungi in Plant Species: Traditional ...
I think that Fluorescence in situ hybridization (abbreviated FISH) will help you you.
Thank you Alex and Mohamed. I will check on these techniques.
Best wishes,
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