We are planning to design primer/probe targeting the conserve sequence of a specific gene. We are struggling to find the conserve region. Which approach/tool is applicable to find the conserve region of a specific gene?
If you are looking for regions that are conserved in the viral genome you will have to preform a multiple alignment of different isolates of the virus you study, unless someone have already preformed this and you can find the list of conserved regions in the literature.