I'm designing CAR-T cells targeting solid tumors. But I was stumped entirely by the designing of the gene sequences for CAR-T. I want to design anti-CEA and anti-MLSN CAR-T cells. I can find the gene sequences of them but I don't know how to choose. I found some patents of CAR-T cells, but some intracellular regions such as CD28 and 4-1BB. Even if the same molecule they used, the gene sequences are different. Some of them just have several different amino acids, some have a lot. I want to know the steps to choose the sequences of anti-CEA and anti-MLSN scFv and the intracellular regions for CAR-T cells. And how could I make sure the gene sequence? I hope for the reply. Thank you.
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Hello. This is my question too. Can you help me with your information and experiences?
thank you
Respectable Wang Mengfei, This is a fascinating question. Unfortunately, I am not an expert in this field. However, I believe this publication may provide you some useful information. The links is:
https://www.sciencedirect.com/science/article/pii/S1471490615001519
https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201700097
https://www.nature.com/articles/s41419-018-0278-6
https://www.sciencedirect.com/science/article/pii/S0304383518302015
https://www.nature.com/articles/s41467-019-13880-1?elqTrackId=d7fd5cabf609432c8419acc6ddb9d171
https://onlinelibrary.wiley.com/doi/abs/10.1111/imr.12773
Best wishes for your upcoming research.
Thanks a lot