How to design a sgRNA (for CRISPR CAS), if the web servers don´t have as "target organism" my microorganism of interest?

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Omar Tovar

Hi Mixtli

You can download the trial of Geneious software. It allows you to design your sgRNA easily and you can use any genome as well. Just check the tutorials.

Hope it helps!

Kasidet Hiranniramol

Some (free) online resources will have custom sgRNA design options. The one I’m familiar with is WU-CRISPR: http://crispr.wustl.edu

You can paste in the sequence of the gene you want to edit.

C K Gomathy

hi,

check this link:

https://www.researchgate.net/topic/Webservers