How to design a probe and primers for different SNP sites for this gene manually. I see the SNP in NCBI site in internet but I do not know how to do?

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António Maximiano Fernandes

We did it using sigma oligoarchitech

you can get both probe and primers. You can get primers and probe for light cycler probe, taqman. scorpion

Christian Cole

Also be aware of any other common SNPs which are nearby to SNP of interest. If they overlap any primer sites you could end with lots of failed PCRs.