How to denature pre-mRNA for RT-PCR ?

10 October 2013 1 3K Report

mRNA are easy to denature but denaturation of pre-mRNA requires additional steps.

I have tried heating at 70C and 80C for 10 minutes prior to cDNA synthesis, but it hasn't worked yet, can someone suggest another method?

Leszek Blaszczyk

Did you use RNAse H to degrade RNA from RNA:DNA hybride? It helped me.

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