To prove the bio degradation of biopolymers, what are all the best methods? what can accelerate degradation?
I would need more details to give a specific answer to your question. In general, what is needed to prove biodegradation of biopolymers would be the same for the biodegradation of any material. If you do not radio-label the polymer, then you next best approach would be to track the microbial numbers of active degraders, disappearance of parent compound (i.e., biopolymer), the production and subsequent degradation of intermediates, etc. For accelerating degradation, increasing the microbial activity by using more inoculum, use of a consortium versus individual species, use of inducers, etc.
Sau I prepare a thin film or mould of biopolymer, which would be the best way to prove degradation?
Take a molecular weight of bio film before bio degradation and the same film you can dissolve and add mixed culture.
(2) Monitor every two days bacteria counting, if the bacteria growth is increases your
degradation is started.
(3) After one week, again solution casting and prepare the same bio-film, and now take
molecular weight of polymer.
Now you can easily calculate the % of bio degradation.
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