Please send me the protocol for the decontamination of EtBr containing chemical waste, which produced after DNA electrophoresis gel experiments.
Dear Priyanka
Ethidium bromide is mutagenic and moderately toxic and must be handled with care. The powder form is considered an irritant to the upper respiratory tract, eyes, and skin. Ethidium bromide is thought to act as a mutagen because it bonds in double stranded DNA, thereby deforming the molecule. This is believed to block or trip biological processes occurring on DNA. Preparation of stock solutions and any operations capable of generating Ethidium bromide dust or aerosols should be conducted in a fume hood to prevent inhalation. Nitrile gloves, a lab coat, and eye protection should be worn at all times, as with working with any hazardous material.
Electrophoresis gels or agarose gels containing ethidium bromide are a common waste stream . All gels containing ethidium bromide are collected and disposed of through EH&S as chemical waste. The containers are provided by EH&S, must be labeled as “Non-hazardous Waste – Ethidium Bromide Gels & Debris” When the container is approximately 75 % full, submit an online Chemical Waste Pickup Request. Ethidium bromide solutions and buffer solutions containing ethidium bromide should be filtered, decontaminated, or destroyed using one of the methods attached herewith
For detail Please find attached Herewith Ethidium Bromide: Disposal, Decontamination, and Destruction pdf file
few more
http://www.ehrs.upenn.edu/programs/environ/waste/ebpolicy.html
https://www.ohio.edu/riskandsafety/docs/Ethidium%20Bromide%20Waste%20Disposal.pdf
http://www.towson.edu/AdminFinance/facilities/ehs/documents/GuidelinesforSafeUseDisposalofEthidiumBromide2.pdf
http://www.ehs.pitt.edu/assets/docs/ethidium-bromide-disposal.pdf
http://www.ncku.edu.tw/biochem/E-S-H/SMOU-Cleanup_of_Ethidium_Bromide.pdf
We are taking all the precaution possible in our lab, but I want to know the procedure before disposal of the waste bin, like some say acid alkali treatment. If any one know the detail protocol the percentage of acid or base before dumping it, please tell me.
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