I have isolated the fungi from infected Capsicum leave sample, culture in Rye media.
In media it was producing long white mycelial growth at 16oC incubation. While increasing temperature to 28oC or at exposing to visible light, the culture turns to yellow.
Microscopic examination at 40X I see the following structure. I can't I identify the fungi.
Iam not exactly sure...looks like chains of conidia of powdery mildew fungi
These are not conidia of Powdery mildews. These are may be Chlamydospores.
why don't you go for molecular identification of isolated fungi using 18 s r DNA
Priyanka
If you consider, time, money for culture media, and effort, it is easier and cheeper to sequence your sample and get a good idea of what it is. The best region for species identification of fungi is the ITS. However if it is something rare or not represented in public data bases the 18s is a good option
Hi Roy: This is Pythium aphanidermatum that caused root rot disease on capscium .
Hello,
If there are a few photographs of macro and micro parts will help in identification.
The family may Pleosporaceae
I wish you good work.
Fungal taxonomy and identification of most fungi is based on growth on PDA, Czapek solution agar and others at 28 - 30 degree C., cultural and morphological characters. Where taxonomic problems arise molecular methods will help in delineating the species. Fungal cultures are easy to identify if one follows proper methods which are very simple and cheap. With the present knowledge, over one lakh species of fungi are known. Only familiarity with the fungi is needed instead of complicated procedures in bacteriology. Identification of fungi on cultural and morphological methods will never go wrong except in a few cases of fungi which are complicated ; where molecular/DNA methods help. With the present knowledge and techniques DNA and molecular methods may lead to wrong conclusions due to technical problems arising in the process.
totally agree with Dr. Anisetti Thammayya. I also faced such problems in some fungal identification.
So what can I do for its identification! For molecular identification should I go for 18s sequence. Or what else I must do for it's identification?
No, don't use 18S. It's way too invariable, it may not even place your isolate in an order.
Use ITS. If it has low similarity with characterised species, use LSU for a proper phylogenetic placement.
Dear X. V. Campos Sir
Thank u for your answer. But I need detail explanation in molecular detection, as it first time I'm going to do it. Can you please give me some publication about the procedure.
Check the methods in the paper below. It's not a description of the method but an example of use.
If you don't have the required equipment, most DNA sequencing services usually provide the option of submitting culture plates and they do the DNA extraction and pre-sequencing PCR for you. Nowadays it's not expensive and if it's something you are not familiar with, it can be a way to avoid all the trouble.
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