Hello.
I've prepared Golgi-Cox slides of hippocampus and prefrontal cortex. I'm planning to analyze total dendritic spine density and density of several types of spines separately by Imaris Software.
My understanding is that Imaris requires 3D image of neuron to conduct spine analysis so now I'm thinking of creating 3D image by ImageJ, which I can now use.
I can get images of neurons at 40x magnification and additional digital zoom up to 3.0x (please see the attached file). Are there any methods to create 3D images of by ImageJ ?
I've tried everything I could but failed.
I would appreciate your kind reply. Thank you.
Hi Takaki Murakami . You will need images of optical sections of your slides to create a 3D image. If you have this, check out my video tutorials on using Fiji/ImageJ for 3D reconstruction:
https://youtu.be/i-Ta4vCRW2s
Dear Takaki Murakami
Have a look:
1. https://www.nature.com/articles/s41598-018-37377-x
2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7243675/
3. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0084557
4. https://www.frontiersin.org/articles/10.3389/fnana.2020.585513/full
Kind Regards
Qamar Ul Islam
Aparna Sathya Murthy
Johanna Marie Dela Cruz
Qamar Ul Islam
Sorry for late reply. Thank you for sharing the information and papers. I'll try it.
This is the first time I ask a question in ResearchGate, so I'm not sure this is correct way to reply, anyway I really appreciate all of your supports.
Thank you.
Best regards
Murakami
To create a 3D image, you need a z-stack image (image of your sample captured at different optical planes). If you have additional images at the various planes, you can manually combine the images to create a z-stack. This z-stack can be projected into 3D. I hope this helps.
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