I'm a PhD student and for my experiment I need to create a luminescent/CFU curve using a plate reader spectrophotometer (it can read luminescent signal). Because I didn't find it in any paper (probably I didn't find the right paper) I would like to have some advice.
I was thinking to culture my Vibrio harveyi in BOSS media. After that to create a serial dilution using 0.85% NaCl solution. From each dilution I will plate 0.1 ml in Boss agar and 0.2 will be used to read the luminescent signal using 96 white well plate.
Another option would be to culture my Vibrio harveyi in BOSS media. After that to create a serial dilution using the BOSS media. From each dilution I will plate 0.1 ml in Boss agar and 0.2 will be used to read the luminescent signal using 96 white well plate up to 24 hours every 30 min.
Anyone with experience in this can give me an advice?
Hi there.
So, your question is wether use saline or medium for the dilutions?! The second option makes more sense. You would read the same dilutions you are using to make the CFU right? Don't forget t to correct the differences in the volume after reading the results, as you are using twice the volume for luminescence then the CFU.
Hi Gustavo,
Thanks for your answer, So use the Boss media is more appropriate. Yes i will plate 0.1 ml of x dilution and read 0.2 ml of the same dilution.
thanks
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