Several journals mentioned counting filamentous cyanobacteria in CFU, but in my experience my culture (Nostoc) is always in aggregation form, even in liquid medium. Can anyone tell me the technique of counting filamentous cyanobacteria?
Counting CFU of filamentous cyanobacteria is useful when estimating their abundance in soil using soil suspension dilutions and the plating method. Its not adequate for laboratory cultures of cyanobacteria.
See; Roger PA, Jimenez R, Santiago-Ardales S (1991) Methods for studying blue-green algae in ricefields: distributional ecology of the organisms, sampling strategies and estimation of abundance. Int. Rice Res. Paper Series n° 150, 19 pages. The International Rice Research Institute, P. O. Box 933, Manila, Philippines.
Available on this site.
1. dilute the inoculant
2. plate in agar plate with your standardized medium (BG11)
3. after few days colony will appear.
4. count the number of colony of each strains
5. multiply and calculate according to your dilution factor
this is CFU
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