Hello,

I'm doing an enzymatic reaction with a substrate with the following molecular weight

Thyroxine MW: 776,87 ± 0,0143 g/mol

The product of the reaction is Triiodothyronine that as a lower molecular weight than the substrate because you have a loss of one iodine

Triiodothyronine MW: 650,9735 ± 0,0143 g/mol

The calculus that I was doing is the following

note: I used radiolabelled product to monitor the reaction with a radiometer (* is for radiolabelled)

[T3*] (test tubes) = T3* area (Test tubes) X [T4*](initial substrate concentration) / T4* area (in control tubes)

I'm looking for a way to correct the result for T3* concentration based on the fact the the substrate and its product doesn't have the same molecular weight so 1mole of T4 is not equal to 1mole of T3

Anyone can help ?

Thanks