How to correct misalignment of Optical system of Operating microscope ?

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Use of LLM will replace the Human Resource?

heavy use of Large Language Model will going to change the working style of organization?

23 June 2024 7,451 3 View

I am aiming to detect alarmins/DAMPS from cell lysate with untargetted mass spectrometry. Is this possible?

alarmins im interested in include: HMGB1 and S100 proteins amongst others Will other cell lysate proteins mask the signal from the alarmins im interested in?

30 May 2024 8,127 0 View

Can be publish any paper through research gate?

24 March 2024 10,037 3 View

Are there indices that encapsulate both rainfall and temperature influences on river basins?

Hello, I'm studying rainfall and temperature's impact on river basin water quality. Are there any indices that effectively capture both rainfall and temperature? I have only temperature and...

10 March 2024 7,224 3 View

Has anyone ever performed MASW using DAS acoustic sensing approach , as traditionally only geophone sensor are utilized?

Has anyone ever performed MASW using DAS acoustic sensing approach , as traditionally only geophone sensor are utilized. If so the answer is yes, how did they manage to acquire the datasets

29 February 2024 1,137 0 View

Is role of sodium bicarbonate under rated in cardiopulmonary resuscitation ?Does it need to be added in ACLS protocol ?

Resuscitation drugs become ineffective during severe metabolic acidosis ...why can't bicarbonate be a part of ACLS protocol

22 January 2024 5,099 3 View

What spectroscopy/spectrometry technique is best suited for quantitative metabolomic profiling / unbiased biomarker discovery(at low concentrations)?

I am looking to quantify metabolites released into cell media, especially alarmins/DAMPS and cytokines. The concentrations of these inflammatory proteins will be on the nanograms/ml or nanomolar...

03 January 2024 1,628 0 View

Electrolytic Conductivity Measurement using DC and AC techniques?

What are the factors affecting the measurement of electrolytic conductivity using DC and AC techniques? What are the challenges and precautions related to electrolytic conductivity measurement?...

26 June 2023 5,507 2 View

Can FBS, kept at 24-26 degree Celsius for about 16 to 18 hours be used for cell culture?

Will that be stable enough ? how can we check for its stability and efficacy?

14 June 2023 8,742 1 View

Is anybody know constitutive equation of couple stress fluid in vectorial form?

Couple stress fluid constitutive relation in vectorial form

08 June 2023 9,525 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Why do exism movements become permanent dictatorship threats within liberal democracy thinking under majority rule-independent rule of law system?

Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...

04 August 2024 8,125 3 View

How movement of energy in an ecosystem different from chemical cycling & movement of matter through an ecosystem differ from movement of energy?

How are the movement of energy in an ecosystem different from chemical cycling and movement of matter through an ecosystem differ from the movement of energy?

03 August 2024 6,134 0 View

Why cycling of matter important to life on Earth & movement of matter through an ecosystem different than movement of energy through an ecosystem?

Why is the cycling of matter important to life on Earth and movement of matter through an ecosystem different than the movement of energy through an ecosystem?

03 August 2024 5,061 3 View

How is matter moved through an ecosystem and movement of energy different from the movement of nutrients through ecosystems?

03 August 2024 4,859 0 View

How the movement of matter is different from the flow of energy and what best describes how matter moves through an ecosystem?

03 August 2024 3,664 2 View

Can't spin-orbit coupling(SOC) be included in optical properties calculation in CASTEP?

I am currently using CASTEP version 2020. When I want to run optical properties calculations with spin-orbit coupling(SOC), I encounter the following error in a pop-up window before the job gets...

02 August 2024 2,890 0 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

Rajib Biswas

Have a look at these articles

a) https://www.biorxiv.org/content/biorxiv/early/2018/05/01/312207.full.pd

b) https://www.osapublishing.org/viewmedia.cfm?uri=ao-55-22-5967&seq=0

c) zeiss-campus.magnet.fsu.edu/.../pdfs/Davidson_Abramowitz_Optical_Microscopy.pd

d) Article Binary phase masks for easy system alignment and basic aberr...

e) https://wp.optics.arizona.edu/optomech/wp-content/.../sites/.../Article-for-W.Cai_.pd

Mohammad Soroosh

The following links are very useful:

https://onlinelibrary.wiley.com/doi/pdf/10.1002/9783527671595.app1

http://post.queensu.ca/~chinsang/lab-protocols/proper-alignment-of-the.pdf

Shahriar Rashvand

Depends on what you are working with?

are you talking about the optical path or the beam inside the microscope itself?

Satish Narula

Both