Misalignment may happen after movement of arms
Have a look at these articles
a) https://www.biorxiv.org/content/biorxiv/early/2018/05/01/312207.full.pd
b) https://www.osapublishing.org/viewmedia.cfm?uri=ao-55-22-5967&seq=0
c) zeiss-campus.magnet.fsu.edu/.../pdfs/Davidson_Abramowitz_Optical_Microscopy.pd
d) Article Binary phase masks for easy system alignment and basic aberr...
e) https://wp.optics.arizona.edu/optomech/wp-content/.../sites/.../Article-for-W.Cai_.pd
The following links are very useful:
https://onlinelibrary.wiley.com/doi/pdf/10.1002/9783527671595.app1
http://post.queensu.ca/~chinsang/lab-protocols/proper-alignment-of-the.pdf
Depends on what you are working with?
are you talking about the optical path or the beam inside the microscope itself?
Both
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