I am characterizing unknown proteinous- fibrous material by AFM. The sample is deposited directly on the surface and fibrils are surrounded by small protein segments.
Now, I can use AFM images and calculate volume of the small protein segments.
How can I than use volume value and average protein density to get molecular mass of unknown protein segments from my AFM image? Is it Volume x Density?
Any suggestion is much appreciated!
This paper gives a series of values for the density of a protein
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2286542/
By a standard curve using proteins of known MWs, as with anything else. That should give as good results as gel filtration chromatography.
I agree with Tomas, you have to prepare a standard curve. For further information, please see the material and methods section of my publication attached.
Article XPB helicase regulates DNA incision by the Thermoplasma acid...
Thanks you all for taking time to answer my question. However, standard curve is not an option in my case. I have my unknown proteinous material on the substrate and wonder is it possible to use AFM images measure volume of proteins and with density determine molecular mass.
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