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11 January 2021 3,284 3 View

How can we count the number of edges in an edge detected image using canny/sobel algorithm in MATLAB?

I need to process an image for edge detection and then want to count the number of edges in that image.

29 December 2020 5,594 2 View

Which are the vendors who sell commercial SWIR (NIR-II) dyes?

I am searching to find commercial vendors who sell SWIR dyes. I could only find Nirmidas Biotech which sells different NIR-II dyes!

23 December 2020 5,002 3 View

How to get doi of presentation ?

doi of your presentation in the conference

23 December 2020 9,370 2 View

Precipitation of Pcl from polymeric solution ?

We working on PCL nano particles 1).Ketoprofen (10mg)+ dmso(5ml) Pcl (10mg)+ DCM(5ml) 2).Water 10 ml+ 10 mg When we mix both 1+2 after solublization (400 rpm) Why precipitation of polymer occurs?

09 December 2020 5,033 11 View

How to induce doxorubicin resistance in MCF-7 and MDA-MB-231?

I want to establish DOX/R cell line in vitro for the research study.If any one suggest the protocol then it would be a great help.

03 December 2020 9,795 5 View

What is the difference between weak from Ritz method and variational form Ritz method while solving a boundary value problem ?

What is the difference between weak from Ritz method and variational form Ritz method while solving a boundary value problem ? Can all differential equations take variational form ?

18 November 2020 2,341 2 View

What could be the reason for a broad peak of ADC(Antibody Drug Conjugates) using HIC-UV method (Hydrophobic Interaction Chromatography)?

Hi, We are working on DAR (Drug to Antibody Ratio) estimation of ADC using the following HIC-UV method. For some of the unconjugated mab we getting a sharp peak but for our desired mab we are...

15 November 2020 7,359 0 View

Can SEM-EDS give a chemical formula?

I have attached an excel file with the quantification of a mineral I conducted EDS on. I have more excel files of minerals too as I know EDS spectra for only 1 sample will certainly not be...

03 November 2020 6,899 9 View

Can TBDMS protecting group be cleaved under 25% TFA/DCM?

I performed a boc deprotection via 25% TFA/DCM (1 hr) without any workup, which should yield TFA salt as a product. My compound also had a TBDMS group on it. But when I took an NMR, the amine peak...

21 October 2020 4,470 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View