What is the best way to make an in-house database with allele frequency ?

Hi, I am currently working on making a variants database. I generate seperate VCF files for each samples them and merge them using VCFtools (using mergeVCF command) to make a multisample VCF...

31 December 2017 8,185 6 View

Sambamba-sort problem (not enough data in stream) ?

Hello, I'm getting an error: "sambamba-view: not enough data in stream" by running sambamba-sort, below my script : PS: I get this error only for some bam files but for all !! "sambamba view -t...

01 February 2017 4,910 0 View

How to create a test predictor with the genotype case/control study (genetic association) ?

Hello,I have a Case / Control genotype study (one qualitative phenotype). so I carried a genetic association analysis with PLINK (logistic) and I've identified hundreds of SNPs with very low Pval...

07 August 2016 1,404 4 View

How to calculate Gene-Gene Pearson correlation ?

31 December 2015 7,063 13 View

What option do you usually use with featureCounts to have count according to isoform ?

09 October 2015 5,000 1 View

How can I make a Polytomous analysis to define clusters in RNA-seq count data?

03 April 2015 5,340 3 View

How can I calculate the average values in dataframe with R?

I have a dataframe with a first column contains the gene symbol and the others column contains an expression values. the Column of symbol can contain the same symbol more then one time. So I would...

02 March 2015 1,980 5 View

How can I do clustering with RNA-Seq data ?

I have Cuffdiff (--no-diff) output tables and I want to perform clustering : 1) better to use Count or FPKM ? 2) Are "standard" clustering algorithms appropriate for RNA-Seq data due to its...

02 March 2015 7,399 23 View

Does anyone know how to estimate the inflection point using r?

01 February 2015 6,469 5 View

Is there a tool for converting a variable step wig to a fixed step wig?

01 February 2015 888 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View