I try to use the line profile, but it just intersects with a few spots in the whole SAED pattern. Is there any way to sum all the spot intensity as a function of distance in reciprocal space?
I think you are interested in measuring the radial intensity profiles of rings similar to what we have in XRD. I think you could try imageJ script in order to do so. Check the link below: https://imagej.nih.gov/ij/plugins/radial-profile.html
You can use a DM script "RDFTools" to generate a line intensity profile of any SAED pattern. You can download it from http://www.dmscripting.com/rdftools.html.